p21 / WAF1(WA-1), 1mg/mL

Description: This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, BSA-free

Concentration: 1 mg/mL

Storage buffer: PBS, no BSA, no azide

Product Origin: Animal - Mus musculus (mouse)

Clone: WA-1

Entrez Gene ID: 1026

Immunogen: Human recombinant p21 protein

Expected AB Applications: Flow (intracellular) (published for clone)/IF (published for clone)/WB (published for clone)

Verified AB Applications: IHC (FFPE) (verified)

Z-Antibody Applications: Flow, intracellular (published)/IF (published)/IHC, FFPE (verified)/WB (published)

• Artikelnummer: BTMBNUM0043-50
• Hersteller: Biotium
• Hersteller Artikelnummer: BNUM0043-50
• Green Labware: Nein
• Verpackungseinheit: 50 uL
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus), Primate, Monkey (Primate)
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Western Blotting, Flow Cytometry, Immunohistochemistry
• Isotyp: IgG1 kappa
• Wirt: Mouse