p33ING1 protein-HIS Epitope

Application Note: p33ING1 Protein is stored in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. p33ING1 Protein is suitable for use in Western Blot. Expect a band approximately ~38kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 0.2 µg/µL

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Recombinant full-length human p33ING1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >85% by densitometry.

Background: p33ING1 displays characteristics of a tumor suppressor protein and can induce cell growth arrest and apoptosis. p33ING1 is a nuclear protein and acute expression of p33 ING1 inhibits cell growth. p33ING1 physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Mutation of p33ING1 gene occurs in neuroblastoma cells and reduced expression is observed in some breast cancer cell lines (1). It has been proposed that p33ING1 can act as a potent growth regulator in normal and in established cells and plays a role as a candidate tumor suppressor gene whose inactivation may contribute to the development of cancers (2). p33ING1 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

Low Endotoxin: No

• Artikelnummer: ROC009-001-S64S
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-S64S
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Konjugat: Unconjugated