p38δ (p38delta), GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: p38δ (SAPK4) is a member of the p38 MAPK family and is activated by chemical and environmental stresses as well as by proinflammatory cytokines. p38δ has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines (1). MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase (2).

Description: Recombinant human p38δ (p38delta), full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_002754

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: O15264

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Goedert, M. et al: Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6); comparison of its substrate specificity with that of other SAP kinases. EMBO J. 16: 3563-3571, 1997.
2. Kumar, S. et al: Novel homologues of CSBP/p38 MAP kinase: activation, substrate specificity and sensitivity to inhibition by pyridinyl imidazoles. Biochem Biophys Res Commun. 1997 Jun 27;235(3):533-8.

• Artikelnummer: BPS40145
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40145
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect