PAK2 protein-GST fusion

Application Note: PAK2 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. PAK2 Protein is suitable for use in Western Blot. Expect a band approximately ~87kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 0.1ug/ul

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Recombinant full-length human PAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Background: PAK2 proteins is a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell (1). The CDC42 and RAC1 induce autophosphorylation of PAK2, which stimulates sustained phosphorylation of other substrates (2). PAK2 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Neurobiology, and Ser/Thr Kinases research.

Low Endotoxin: No

• Artikelnummer: ROC009-001-S78S
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-S78S
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Konjugat: Unconjugated