PAK2 Recombinant

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Encompassing Amino Acids: 227-524

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK2 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm

Background: PAK proteins is a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. The CDC42 and RAC1 induce autophosphorylation of PAK2, which stimulates sustained phosphorylation of other substrates.

Description: Human PAK2 (GenBank Accession No. NM_002577), a.a. 227-524, MW=33.5 kDa, expressed in E. coli.

Format: Aqueous buffer solution

Formulation: 25 mM Tris-HCl, pH 8, 150 mM NaCl, 10% glycerol, 5 mM DTT

Genbank: NM_002577

Purity: ≥95%

Storage Stability: At least 6 months at -80°C.

Tags: none

Uniprot: Q13177

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Coniglio,S.J., et al., Mol. Cell. Biol. 28 (12), 4162-4172 (2008).
2. Koh,W., et al., J. Cell. Sci. 121 (PT 7), 989- 1001 (2008).

• Artikelnummer: BPS40073
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40073
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK