PAK6 Recombinant

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Encompassing Amino Acids: 385-680

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK5 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm

Background: PAK6 is a Ser/Thr protein kinase that is a member of the PAK (p21-activated kinase) family that are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 is highly expressed in the testis and prostate tissues and interacts with the androgen receptor (AR). In response to androgen, PAK6 cotranslocate into the nucleus with AR. PAK6 is weakly expressed in normal prostate epithelium but the expression is increased in primary and metastatic prostate cancer cells and is further increased in tumors that relapse after androgen deprivation therapy.

Description: Human PAK6 (GenBank Accession No. NM_020168), a.a. 385-680, MW=34.2 kDa, expressed in E. coli.

Format: Aqueous buffer solution

Formulation: 25 mM Tris-HCl, pH 8, 150 mM NaCl, 10% glycerol, 5 mM DTT

Genbank: NM_020168

Purity: ≥95%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: Q9NQU5

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Kaur,R., et al., Prostate 68 (14), 1510-1516 (2008).
2. Hosgood,H.D. et al., Carcinogenesis 29 (10), 1938-1943(2008).

• Artikelnummer: BPS40081
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40081
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK