PARC Antibody

Application Note: Anti-PARC reacts with human and mouse PARC by western blot and immunoprecipitation.  The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others).  To date co-immunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected.  Most cell lines expressing PARC can be used as a positive control.  Researchers should determine optimal titers for other applications.

Concentration Value: 85 mg/mL

ELISA Dilution: 1:2,000 - 1:10,000

Immunohistochemistry Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:1,000

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse PARC.  Cross reactivity with PARC from other sources is not known.

Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes.  PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC100-401-A07
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-401-A07
• Verpackungseinheit: 100 µL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Isotyp: Antiserum
• Human Gene ID: 23113
• Wirt: Rabbit
• Konjugat: Unconjugated