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Encompassing Amino Acids: 2-976 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Various concentrations of PARG were assayed using Fluorogenic Assay Kit (BPS Bioscience #78858) at room temperature with 4 µM TFMU-ADPr (substrate). The fluorogenic product was measured after 1 hour (Excitation: 385 nm / Emission: 502 nm). Enzyme concentration for IC50: 2.5 ng/rxn (~0.9 nM).
Background: PARG is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. It reverses PARP-mediated ADP-ribosylation and plays a role in DNA damage responses. PARG inhibition may prove beneficial in cancer therapy.
Description: Recombinant human PARG (Poly(ADP-ribose) glycohydrolase), full length encompassing amino acids 2-976 (end). The construct contains an N-terminal His-Tag (6xHis). The recombinant protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 610 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.5 mM TCEP, and variable imidazole
Genbank: NM_003631.5
Host Cell Line: Sf9 cells
Purity: ≥70%
Storage Stability: At least 6 months at -80°C.
Uniprot: Q86W56
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)