PARP11 Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Background: PARP11 is known to catalyze NAD-dependent ribosylation.

Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt

Description: The PARP11 Chemiluminescent Activity Assay kit is designed to measure PARP11 activity for screening and profiling applications. The key to the PARP11 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP11 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP11 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: The PARP11 assay kit comes in a convenient 96-well format, with purified PARP11 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

• Artikelnummer: BPS80561
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80561
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK