PARP11, GST-Tag, His-Tag Recombinant

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Encompassing Amino Acids: 8-338 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted usingBPS's PARP11 Assay Kit (Catalog #80561) at room temperature for 1 hour.

Description: Human PARP11 (GenBank Accession No. NM_020367), a.a. 8-338 (end) with N-terminal GST-tag, MW = 65.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 4 mMglutathione, 20% glycerol, and 3 mM DTT.

Genbank: NM_020367

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q9NR21

Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Rouleau M., et al. J Cell Biochem. 2007 Feb 1;100(2):385-401.
2. Augustin A., et al. J Cell Sci. 2003 Apr 15;116(Pt 8):1551-62.

• Artikelnummer: BPS80511
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80511
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK