PARP15 Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Background: PARP15 (also known as BAL3 or ARTD7) is a transcriptional repressor that exhibits poly(ADP-ribose) polymerase activity.

Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt

Description: The PARP15 Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. The key to the PARP15 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP15 reactions. First, histone proteins are coated onto the wells of the strip-plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP15 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: The PARP15 assay kit comes in a convenient 8-well strip format, with purified PARP15 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
2. Aguiar R.C., et al. 2005 J. Biol. Chem. 280(40):33756-65.

• Artikelnummer: BPS80567
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80567
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK