PARP2, GST-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-583

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted usingBPS's PARP2 Assay Kit (Catalog #80552) at room temperature for 1 hour.

Description: Human PARP2 (GenBank Accession No. NM_005484), a.a. 2-583 with N-terminal GST-tag, MW = 92 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 16 mMglutathione, 20% glycerol, and 3 mM DTT.

Genbank: NM_005484

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q9UGN5

Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Jha, R., et al., Fertil. Steril. 91 (3), 782-790 (2009).
2. Quenet, D., et al., FASEB J. 22 (11), 3853- 3865 (2008).Application References:
1. BMN 673, a novel and highly potent PARP1/2 inhibitor for the treatment of human cancers with DNA repair deficiency (2013)
2. Fragment-based ligand design of novel potent inhibitors of tankyrases (2013)

• Artikelnummer: BPS80502
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80502
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK