PARP7, FLAG-Tag Recombinant

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Encompassing Amino Acids: 400-657 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: >40 pmol/min/µg Assay Conditions: Assay was done according to PARP7 assay kit protocol #80557 at room temperature for 1 hour..

Description: Human PARP7 (GenBank Accession No. NM_015508), a.a. 400-657 (end) with N-terminal FLAG-tag, MW = 31 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3 mM DTT, 20% glycerol and 100 µg/mL FLAG peptide.

Genbank: NM_015508

Storage Stability: At least 6 months at -80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Tags: N-terminal FLAG-tag

Uniprot: Q7Z3E1 Q0PNE2

Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Rouleau M., et al. J Cell Biochem. 2007 Feb 1;100(2):385-401.2. Augustin A., et al. J Cell Sci. 2003 Apr 15;116(Pt 8):1551-62.

• Artikelnummer: BPS80527
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80527
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK