PD-1 (CD279), Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function.

Assay Conditions: BPS Cat. 72005protocol was followed. Briefly, 50 µl of 2µg/ml PD-L1 was coated onto plates. 20 µlof 0.5 µg/ml PD-1, Biotin-labeled wasallowed to bind at room temp. for 2 hours.Strep-HRP was added and allowed toincubate at room temp for 1 hr, followed byHPR chemiluminescent substrate additionand plate reading.

Description: Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a 25-167, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avitag ™ expressed in a HEK293 cell expression system and enzymatically biotinlabeled using Avi-tag™ technology. MW = 44.5 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

Format: Aqueous buffer solution

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Genbank: NM_005018

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™

Uniprot: Q15116

Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.

Biosafety Level: Not applicable (BSL-1)

References: 1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.

• Artikelnummer: BPS71109-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 71109-2
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK