PD-1:PD-L1(Biotinylated) Inhibitor Screening Colorimetric Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: This kit is useful for screening for inhibitors of PD-1 binding to PD-L1.

Description: Cell signaling through the PD-1 receptor upon binding the PD-L1 ligandattenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL1[Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors ofPD-L1 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L1 for streptavidin-HRP.Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated ona 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated withstreptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, whichcan then be measured using a UV/Vis spectrophotometer microplate reader.

Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled PD-L1, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.

• Artikelnummer: BPS72016
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 72016
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK