PD-1:PD-L2 Homogeneous Assay Kit

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Applications: Useful for screening for inhibitors of PD-1 binding to PD-L2

Contraindications: Only limited amounts of DMSO can be included, as it has been shownto disrupt PD-1:PD-L2 interaction. Avoid green and blue dyes that absorb light in theAlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of thepotent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction dueto the presence of excess biotin and iron in this medium. MEM, which lacks these components,does not affect AlphaScreen assays.

Description: The PD-1:PD-L2 Homogeneous Assay Kit is designed to measure theinhibition of PD-1 binding to PD-L2. With this kit, only three simple steps on a microtiterplate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated withthe biotinylated PD-L2 for 60 minutes. Next, acceptor beads are added, then donor beads,followed by reading the Alpha-counts.

Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: The PD-1:PD-L2 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated PD-L2, FLAG-tagged PD-1, and assay buffer to perform a total of 384 reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Molnar, E. et al. Proc. Natl. Acad. Sci. U.S.A. 2008; 105: 10483-10488
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008; 26: 677-704.

• Artikelnummer: BPS72015
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 72015
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK