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Encompassing Amino Acids: 19-239
Application: Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Assay Conditions: PD-1-PD-L1 binding activity, measured using the using the PD-1:PDL1 [Biotinylated] Inhibitor Screening Assay Kit, BPS Bioscience, Catalog #72003.
Description: Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the Cterminus with the Fc portion of human IgG1, with C-terminal Avi-tag™ expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 54 kDa.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_014143
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
Uniprot: Q9NZQ7
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Freeman, G.J., et al., J. Exp. Med. 2000 Oct 2;192(7):1027-34.2. Dong, H., et al., Nat Med. 1999. Dec;5(12):1365-9.