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Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Description: Mouse secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_021893, a.a. 18-238, fused at the C-terminus to the Fc portion of mouse IgG2a and with a Cterminal Avi-tag, expressed in a HEK293 cell expression system. This protein was biotinylated using AviTag™ technology. MW = 54 kDa. Note: this protein runs at an apparent higher molecular weight due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Genbank: NM_021893
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Mouse IgG2a), C-terminal Avi-tag™
Uniprot: Q9EP73
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Freeman, G.J., et al., J Exp Med. 2000; 192(7):1027-34.
2. Dong, H., et al., Nat Med. 1999; 5(12):1365-9.