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PDE10A (Rat), GST-tag Recombinant
PDE10A (Rat), GST-tag Recombinant
Artikelnummer
BPS60102
Verpackungseinheit
5 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 449-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, and 2.5 kU 5?- nucleotidase, 37°C, 20 min. Quantified by 5'-nucleotidasecleaving the 5'-AMP product and releasing the phosphate group which is detected byMalachite Green Reagent.
Description: Rat PDE10A GenBank Accession No. NM_022236), a.a. 449-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_022236
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9QYJ6
Unit Definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006).
2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).
Encompassing Amino Acids: 449-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, and 2.5 kU 5?- nucleotidase, 37°C, 20 min. Quantified by 5'-nucleotidasecleaving the 5'-AMP product and releasing the phosphate group which is detected byMalachite Green Reagent.
Description: Rat PDE10A GenBank Accession No. NM_022236), a.a. 449-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_022236
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9QYJ6
Unit Definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006).
2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).
| Artikelnummer | BPS60102 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60102 |
| Green Labware | Nein |
| Verpackungseinheit | 5 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
