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PDE10A1, GST-tag Recombinant
PDE10A1, GST-tag Recombinant
Artikelnummer
BPS60099
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-789(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/µl PDE10A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Human PDE10A1 (GenBank Accession No. NM_001130690), a.a. 2-789(end) with N-terminal GST-tag, MW=116 kDa, expressed in Sf9 cells via a Baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 20 mMglutathione, 3 mM DTT and 10% glycerol.
Genbank: NM_001130690
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9Y233
Unit Definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).Product Specific Reference(s): 1. Demirbas, D., et al., PLoS One. 8(8), (2013).
2. Wang, Z., et al., J Sex Med. 10(11), 2790-7 (2013).
Encompassing Amino Acids: 2-789(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/µl PDE10A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Human PDE10A1 (GenBank Accession No. NM_001130690), a.a. 2-789(end) with N-terminal GST-tag, MW=116 kDa, expressed in Sf9 cells via a Baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 20 mMglutathione, 3 mM DTT and 10% glycerol.
Genbank: NM_001130690
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9Y233
Unit Definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).Product Specific Reference(s): 1. Demirbas, D., et al., PLoS One. 8(8), (2013).
2. Wang, Z., et al., J Sex Med. 10(11), 2790-7 (2013).
| Artikelnummer | BPS60099 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60099 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
