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PDE11A4 Assay Kit
PDE11A4 Assay Kit
Artikelnummer
BPS60411
Verpackungseinheit
96 rxns.
Hersteller
BPS Bioscience
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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: Phosphodiesterases (PDEs) are involved in the dynamic regulation ofcAMP and cGMP signaling. PDE11A has been observed in skeletal muscle, prostate,testis, salivary gland, thyroid gland, and liver. PDE11A may play role in the CNS with anassociation to major depressive disorder. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeledcyclic monophosphates. Beads selectively bind the phosphate group in the nucleotideproduct. This increases the size of the nucleotide relative to unreacted cyclicmonophosphate. In the polarization assay, dye molecules with absorption transitionvectors parallel to the linearly-polarized excitation light are selectively excited. Dyesattached to the rapidly-rotating cyclic monophosphates will obtain random orientationsand emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-beadcomplexes will not have time to reorient and therefore will emit highly polarized light.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PDE11A Assay Kit is designed for identification of PDE11A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE11A to thebinding agent. The key to the PDE11A Assay Kit is the specific binding agent. Using this kit, only two simple steps on amicrotiter plate are required for PDE11A reactions. First, the fluorescently labeled cAMPis incubated with a sample containing PDE11A for 1 hour. Second, a binding agent isadded to the reaction mix to produce a change in fluorescent polarization that can thenbe measured using a fluorescence reader.
Storage Stability: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PDE11A inhibitor screening assay kit comes in a convenient 96-well format,including purified PDE11A enzyme, fluorescently labeled PDE11A substrate (cAMP),binding agent, and PDE assay buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Wong. ML., et al. Proc Natl Acad Sci U S A. 2006; 103(41): 15124-9.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: Phosphodiesterases (PDEs) are involved in the dynamic regulation ofcAMP and cGMP signaling. PDE11A has been observed in skeletal muscle, prostate,testis, salivary gland, thyroid gland, and liver. PDE11A may play role in the CNS with anassociation to major depressive disorder. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeledcyclic monophosphates. Beads selectively bind the phosphate group in the nucleotideproduct. This increases the size of the nucleotide relative to unreacted cyclicmonophosphate. In the polarization assay, dye molecules with absorption transitionvectors parallel to the linearly-polarized excitation light are selectively excited. Dyesattached to the rapidly-rotating cyclic monophosphates will obtain random orientationsand emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-beadcomplexes will not have time to reorient and therefore will emit highly polarized light.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PDE11A Assay Kit is designed for identification of PDE11A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE11A to thebinding agent. The key to the PDE11A Assay Kit is the specific binding agent. Using this kit, only two simple steps on amicrotiter plate are required for PDE11A reactions. First, the fluorescently labeled cAMPis incubated with a sample containing PDE11A for 1 hour. Second, a binding agent isadded to the reaction mix to produce a change in fluorescent polarization that can thenbe measured using a fluorescence reader.
Storage Stability: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PDE11A inhibitor screening assay kit comes in a convenient 96-well format,including purified PDE11A enzyme, fluorescently labeled PDE11A substrate (cAMP),binding agent, and PDE assay buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Wong. ML., et al. Proc Natl Acad Sci U S A. 2006; 103(41): 15124-9.
| Artikelnummer | BPS60411 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60411 |
| Green Labware | Nein |
| Verpackungseinheit | 96 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
