Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: full length
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE1B Assay Kit, Cat. # 60311. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl
2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE1B at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST-tag, MW= 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 380 mM NaCl, 1 mM DTT, 10% glycerol
Genbank: NM_000924
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q01064
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5?-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Bender A.T. and Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 103 (2), 460-465 (2006).
2. Bender A.T. et al., Proc. Natl. Acad. Sci. U.S.A. 102 (2), 497-502 (2005).Application Reference(s):
Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry (2013)
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