PDE1B (Mouse), GST-Tag Recombinant

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Encompassing Amino Acids: 42-535 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE1B Mouse at 37ºC for 20 min. Quantified by 5ʹ nucleotidase cleaving the 5ʹ-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Description: Mouse PDE1B (GenBank Accession No. NM_008800), a.a. 42-535 (end), with N-terminal GST-tag, MW= 83 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol.

Genbank: NM_008800

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST tag

Uniprot: Q01065

Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3ʹ, 5ʹ-cAMP to 5ʹ-AMP per min at 37°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Spence, S., Biomedical Journal, 321(1),:157-163 (1997).2. Xin, J. et al., Proc. Natl. Acad. Sci. U.S.A. 93: 11236-11241 (1996).

• Artikelnummer: BPS11665
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 11665
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect