PDE2A V4 (Mouse), GST-tag Recombinant

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Encompassing Amino Acids: 2-939 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5ʹ nucleotidase cleaving the 5ʹ-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Description: Murine PDE2A, variant 4 (GenBank Accession No. NM_001243757), a.a. 2-939 (end), with N-terminal GST tag, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 1.6 mM glutathione, 20% glycerol

Genbank: NM_001143848

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Tags: N-terminal GST-tag

Uniprot: O00408

Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3ʹ, 5ʹ-cAMP to 5ʹ-AMP per min at 37°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Sadhu, K., et al., JHC 47(7), 889-894 (1999).2. Iffland, A., et al., Biochemistry 44 (23), 8312-8325 (2005).

• Artikelnummer: BPS60019
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60019
• Green Labware: Nein
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK
• Wirt: Insect