PDE3A (Dog), FLAG-Tag Recombinant

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Encompassing Amino Acids: 343-998 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 0.02% TritonX, and serial dilutions of PDE3A at 37ºC for 20 min. Quantified by 5ʹ nucleotidase cleaving the 5ʹ-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Absorbance was measured at 620 nm.

Description: Dog PDE3A, also known as Phosphodiesterase 3A, CGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A, GenBank Accession No. XP_005637137, a.a. 343-998 (end), with C-terminal FLAG-tag, expressed in a HEK293 expression system, MW = 75 KDa.

Format: Aqueous solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% Glycerol.

Genbank: XP_005637137

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: FLAG-Tag

Uniprot: F1PL79

Warnings: Avoid multiple freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)
2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

• Artikelnummer: BPS100264
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100264
• Green Labware: Nein
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK