PDE3A (Mouse), GST-Tag Recombinant

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Encompassing Amino Acids: 484-1141

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of Mouse PDE3A at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Description: Mouse PDE3A (GenBank Accession No. NM_018779), amino acids 484-1141 end), with N-terminal GST tag, MW = 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 18 mM Glutathione

Genbank: NM_018779

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Tags: N-terminal GST-tag

Uniprot: B2RR84

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)2.Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

• Artikelnummer: BPS60036
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60036
• Green Labware: Nein
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK
• Wirt: Insect