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PDE4A4B, GST-tag Recombinant
PDE4A4B, GST-tag Recombinant
Artikelnummer
BPS60039
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-886(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4A4B at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4A4B, also known as PDE4A isoform 1 (GenBank Accession No. NM_001111307), a.a. (2-end) with N-terminal GST-tag, MW= 124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 4 mM glutathione,3 mM DTT and 20% glycerol
Genbank: NM_001111307
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P27815
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sullivan,M., et al., Journal Biochem. J. 333:693-703 (1998).
2. Huston E, et al., J Cell Sci. 2006 119:3799-810. (2006).
Encompassing Amino Acids: 2-886(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4A4B at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4A4B, also known as PDE4A isoform 1 (GenBank Accession No. NM_001111307), a.a. (2-end) with N-terminal GST-tag, MW= 124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 4 mM glutathione,3 mM DTT and 20% glycerol
Genbank: NM_001111307
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P27815
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sullivan,M., et al., Journal Biochem. J. 333:693-703 (1998).
2. Huston E, et al., J Cell Sci. 2006 119:3799-810. (2006).
| Artikelnummer | BPS60039 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60039 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
