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PDE4B2, GST-tag, His-tag Recombinant
PDE4B2, GST-tag, His-tag Recombinant
Artikelnummer
BPS60042
Verpackungseinheit
5 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE4B2 Assay Kit, Cat. # 60343. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4B2 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4B2 (GenBank Accession No. NM_001037339), full length, with N-terminal GST-tag and C-terminal His-tag, MW=91.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, and 20% glycerol.
Genbank: NM_001037339
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-tag
Uniprot: Q07343
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Arp, J., et al., Mol Cell Biol. 2003 Nov; 23(22):8042-57.
2. Wang, P., et al., Mol Pharmacol. 1999 Jul; 56(1):170-4.Application Reference(s):
Evaluation of the therapeutic index of a novel phosphodiesterase 4B-selective inhibitor over phosphodiesterase 4D in mice (2013)
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE4B2 Assay Kit, Cat. # 60343. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4B2 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4B2 (GenBank Accession No. NM_001037339), full length, with N-terminal GST-tag and C-terminal His-tag, MW=91.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, and 20% glycerol.
Genbank: NM_001037339
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-tag
Uniprot: Q07343
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Arp, J., et al., Mol Cell Biol. 2003 Nov; 23(22):8042-57.
2. Wang, P., et al., Mol Pharmacol. 1999 Jul; 56(1):170-4.Application Reference(s):
Evaluation of the therapeutic index of a novel phosphodiesterase 4B-selective inhibitor over phosphodiesterase 4D in mice (2013)
| Artikelnummer | BPS60042 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60042 |
| Green Labware | Nein |
| Verpackungseinheit | 5 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
