PDE4B3, GST-Tag Recombinant

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Encompassing Amino Acids: 2-721 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE4B3 at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Description: Human PDE4B3 (GenBank Accession No. NM_001037340), 2-721 (end), with N-terminal GST tag, MW=108 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.

Genbank: NM_001037340

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST tag

Uniprot: Q07343

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ahmed, T., et al., Neuroscience. 117(3), 627-638 (2003).2. Millar, J, et al., Science. 310(5751), 1187-1191 (2005).

• Artikelnummer: BPS11675
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 11675
• Green Labware: Nein
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK
• Wirt: Insect