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PDE4C1, GST-tag Recombinant
PDE4C1, GST-tag Recombinant
Artikelnummer
BPS60044
Verpackungseinheit
5 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4C1 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4C1 (GenBank Accession No. NM_000923), full length, with N-terminal GST-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_000923
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q08493
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Wang H. et al., Biochem J. 2007; 408(2):193-201.
2. Sullivan M., Olsen A.S. and Houslay M.D. Cell. Signal. 1999; 11 (10):735-742.
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4C1 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4C1 (GenBank Accession No. NM_000923), full length, with N-terminal GST-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_000923
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q08493
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Wang H. et al., Biochem J. 2007; 408(2):193-201.
2. Sullivan M., Olsen A.S. and Houslay M.D. Cell. Signal. 1999; 11 (10):735-742.
| Artikelnummer | BPS60044 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60044 |
| Green Labware | Nein |
| Verpackungseinheit | 5 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
