PDE5A1 Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Background: Phosphodiesterases (PDEs) play an important role in the dynamic regulationof cAMP and cGMP signaling. PDE5A, also known as cGMP-inhibited phosphodiesterase, hasbeen implicated in cardiovascular function and fertility.

Description: The PDE5A1 Assay Kit is designed for identification of PDE5A1 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE5A to the binding agent. PDE5A catalyzes the hydrolysis of the phosphodiester bond in dye-labeled cyclic guanosine monophosphate. Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic guanosine monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyesattached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.The key to the PDE5A1 Assay Kit is the specificbinding agent. Using this kit, only two simple steps on a microtiter plate are required forPDE5A1 reactions. First, the fluorescently labeled cGMP is incubated with a sample containingPDE5A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a changein fluorescent polarization that can then be measured using a fluorescence reader.

Storage Stability: At least 6 months from date of receipt, when stored as directed

Supplied As: The PDE5A1 inhibitor screening assay kit comes in a convenient 384-well format, including purified PDE5A1 enzyme, fluorescently labeled PDE5A1 substrate (cGMP), binding agent, and PDE assay buffer for 384 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Maurice DH. Front. Biosci. 2005; 10:1221-8.

• Artikelnummer: BPS60351
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60351
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK