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PDE5A1 (Mouse), GST-tag Recombinant
PDE5A1 (Mouse), GST-tag Recombinant
Artikelnummer
BPS60051
Verpackungseinheit
5 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 0.1 mg/mlBSA, 200 µM cGMP, 2.5 kU 5'-nucleotidase, andserial dilutions of mPDE5A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Mouse PDE5A (GenBank Accession No. NM_153422), full length, with N-terminal GST-tag, MW=124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18mM glutathione, 10% glycerol.
Genbank: NM_153422
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8CG03
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Das, A. et al., J. Biol. Chem. 280 (13), 12944-12955 (2005).
2. Champion, H.C.et al., Proc. Natl. Acad. Sci. U.S.A. 102 (5), 1661-1666 (2005).
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 0.1 mg/mlBSA, 200 µM cGMP, 2.5 kU 5'-nucleotidase, andserial dilutions of mPDE5A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Mouse PDE5A (GenBank Accession No. NM_153422), full length, with N-terminal GST-tag, MW=124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18mM glutathione, 10% glycerol.
Genbank: NM_153422
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8CG03
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Das, A. et al., J. Biol. Chem. 280 (13), 12944-12955 (2005).
2. Champion, H.C.et al., Proc. Natl. Acad. Sci. U.S.A. 102 (5), 1661-1666 (2005).
| Artikelnummer | BPS60051 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60051 |
| Green Labware | Nein |
| Verpackungseinheit | 5 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
