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PDE6C, GST-Tag Recombinant
PDE6C, GST-Tag Recombinant
Artikelnummer
BPS60062
Verpackungseinheit
5 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE6C at 37°C for 60 min.Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE6C (GenBank Accession No. NM_006204), full length, with N-terminal GST-tag, MW=125 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM Glutathione, 20% glycerol, and 3 mM DTT.
Genbank: NM_006204
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P51160
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Martinez S.E. et al., J Biol Chem. 2008 Sep 19;283(38):25913-9.
2. Stearns G. et al., J Neurosci. 2007 Dec 12;27(50):13866-74.
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE6C at 37°C for 60 min.Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE6C (GenBank Accession No. NM_006204), full length, with N-terminal GST-tag, MW=125 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM Glutathione, 20% glycerol, and 3 mM DTT.
Genbank: NM_006204
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P51160
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Martinez S.E. et al., J Biol Chem. 2008 Sep 19;283(38):25913-9.
2. Stearns G. et al., J Neurosci. 2007 Dec 12;27(50):13866-74.
| Artikelnummer | BPS60062 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60062 |
| Green Labware | Nein |
| Verpackungseinheit | 5 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
