PDE6C protein-GST fusion

Application Note: PDE6C Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. PDE6C Protein is suitable for use in Western Blot. Expect a band approximately ~125kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 0.1 µg/µL

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Recombinant full-length human PDE6C was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Background: PDE6C or phosphodiesterase 6C, cGMP-specific, cone, alpha prime encodes the alpha-prime subunit of cone phosphodiesterase, which is composed of a homodimer of two alpha-prime subunits and 3 smaller proteins of 11, 13, and 15 kDa. PDE6C protein consists of 22 exons spanning approximately 48 kb of genomic DNA. The intron-exon structure of human PDE6C is very similar to that of rod PDE6B (1). A homologous genetic basis of the murine cpfl1 mutant and human achromatopsia linked to mutations in the PDE6C gene and mutations in PDE6C are associated with cone dystrophy type 4 (COD4) (2). PDE6C Protein is ideal to investigators involved in Signaling Proteins, Cellular Proteins, Cardiovascular Disease, and Inflammation research.

Low Endotoxin: No

• Artikelnummer: ROC009-001-S84S
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-S84S
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Konjugat: Unconjugated