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PDE7A (Rat), GST-tag Recombinant
PDE7A (Rat), GST-tag Recombinant
Artikelnummer
BPS60074
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 147-482(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE7A kit, Cat. # 60370. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of rPDE7A at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Rat PDE7A (GenBank Accession No. NM_031080), a.a. 147-482(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 18 mMglutathione, 10% glycerol, and 3 mM DTT.
Genbank: NM_031080
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: F1LM88
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Han, P., et al. (1998). Genomics Mar 1;48(2):275-6.
2. Yang, G., et al. (2003). J. Immunol. Dec 15;171(12):6414-20.
3. Yamamoto, et al. (2006) Eur. J. Pharmacol. Jul 10;541(1-2):106-14.
Encompassing Amino Acids: 147-482(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE7A kit, Cat. # 60370. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of rPDE7A at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Rat PDE7A (GenBank Accession No. NM_031080), a.a. 147-482(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 18 mMglutathione, 10% glycerol, and 3 mM DTT.
Genbank: NM_031080
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: F1LM88
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Han, P., et al. (1998). Genomics Mar 1;48(2):275-6.
2. Yang, G., et al. (2003). J. Immunol. Dec 15;171(12):6414-20.
3. Yamamoto, et al. (2006) Eur. J. Pharmacol. Jul 10;541(1-2):106-14.
| Artikelnummer | BPS60074 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60074 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
