PDE7B, GST-tag, His-tag Recombinant

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Encompassing Amino Acids: 109-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assayed using BPS's PDE7B kit, Cat. # 60371. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Description: Human PDE7B GenBank Accession No. NM_018945, (a.a. 109-end) with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Genbank: NM_018945

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag, C-terminal His-tag

Uniprot: Q9NP56

Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Gardner,C., et al., Biochem. Biophys. Res. Commun. 272 (1), 186-192 (2000)
2. Sasaki,T., et al., Biochem. Biophys. Res. Commun. 271 (3), 575-583 (2000)Application Reference(s):
Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015)

• Artikelnummer: BPS60071
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 60071
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect