PDGF-BB Recombinant

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Encompassing Amino Acids: 82-190

Amino Acid Sequence: SLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT

Application: Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of BALB/c 3T3 cells proliferation is < 1.0 ng/ml.

Description: Human recombinant PDGF-BB (GenBank Accession No. NM_002608) is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total a.a.) expressed in Saccharomyces cerevisiae.

Endotoxin Level: < 1EU per µg of PDGF-BB as determined by the LAL method.

Format: lyophilized protein

Formulation: Lyophilized from a 0.2 µm filtered 0.02M acetic acid solution, pH 6.5.

Genbank: NM_002608

Purity: ≥98% by SDS-PAGE and HPLC analysis.

Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage Stability: ≥2 years (lyophilized) at -20°C.

Tags: none

Uniprot: P01127

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Szabo, A., et al. Stroke 38 (8), 2247-2253 (2007)
2. Guida, T., et al. Clin. Cancer Res. 13 (11), 3363-3369 (2007)

• Artikelnummer: BPS90800-3
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90800-3
• Verpackungseinheit: 1000 µg
• Mengeneinheit: STK
• Wirt: Yeast