PDGFRβ, His-tag Recombinant

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Encompassing Amino Acids: 558-1106(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: The enzyme reaction was carried out for 1 hour at 30°C in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EDTA, 0.01% BRIJ-35 and 200 µM of ATP. Substrate peptide: Tyr2 peptide from Invitrogen.

Description: Human PDGFRβ (GenBank Accession No. NM_002609), cytoplasmic domain, a.a. 558-1106(end) with N-terminal His-tag, MW= 62 kDa, expressed in Sf9 cells via a Baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Genbank: NM_002609

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P09619

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Claesson-Welsh L. et al. (1988). Mol. Cell. Biol. 8:3476.
2. Heldin C.-H. and Westermark B. (1999). Physiol. Rev. 79:128.
3.Soriano, P. Genes Dev. 1994 Aug 15,8(16):1888-96.
4. Xu, L. et al. Cancer Res. 2005 Jul 1,65(13):5711-9.

• Artikelnummer: BPS40263
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40263
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect