PDK1, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using PDKtide substrate (0.1 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was measured using Kinase-Glo® reagent (Promega)

Background: PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.

Description: Human 3-phosphoinositide-dependent protein kinase (PDK1), GenBank Accession No. NM_002613, full length with N-terminal GST-tag, MW=89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.

Genbank: NM_002613

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: O15530

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Yang, KJ., et al., J Biol Chem. 2008 Jan 18,283(3):1480-91.
2. Kikani, CK., et al., J Cell Biochem. 2005 Dec 15,96(6):1157-62.Application Reference:
PDK1 controls upstream PI3K expression and PIP3 generation (2014)

• Artikelnummer: BPS40080
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40080
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect