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PHGDH, His-tag Recombinant
PHGDH, His-tag Recombinant
Artikelnummer
BPS71079
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1 - 533 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid (Sigma P8877), 1 mM NAD, and various amount of PHGDH in a final volume of 50 µL. Reactions were initiated with the addition of PHGDH, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature.
Description: Human phosphoglycerate dehydrogenase (PGDH), also known as PDG, a.a. 1 - 533 (end) (GenBank Accession No. NM_006623) with C-terminal His-tag, MW = 57.5 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 170 mM imidazole, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_006623
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: O43175
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Mullarky, E., et al., Pigment Cell Melanoma Res. 2011 Dec;24(6):1112-5.
2. Liu, J., et al., J Neurooncol. 2013 Feb;111(3):245-55.
Encompassing Amino Acids: 1 - 533 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid (Sigma P8877), 1 mM NAD, and various amount of PHGDH in a final volume of 50 µL. Reactions were initiated with the addition of PHGDH, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature.
Description: Human phosphoglycerate dehydrogenase (PGDH), also known as PDG, a.a. 1 - 533 (end) (GenBank Accession No. NM_006623) with C-terminal His-tag, MW = 57.5 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 170 mM imidazole, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_006623
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: O43175
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Mullarky, E., et al., Pigment Cell Melanoma Res. 2011 Dec;24(6):1112-5.
2. Liu, J., et al., J Neurooncol. 2013 Feb;111(3):245-55.
| Artikelnummer | BPS71079 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 71079 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
