Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT pan (S473) and Total AKT pan in cell lysates.
Components: Eu-phospho-AKTS473 (20 µL), Acc-phospho-AKTS473 (80 µL), Eu-Total AKT (5 µL), Acc-Total AKT (20 µL), AKT control lysate (500 µL), 5X Lysis Buffer 1 (5 mL), 10X TR-FRET Detection buffer (250 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-AKT pan (S473) & Total AKT pan.
Assay Target Class: Kinase.
SwissProt: P31749 , P31751 , Q9Y243.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).