Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT pan (T308) in cell lysates.
Components: Eu-phospho-AKTT308 (5 µL), Acc-phospho-AKTT308 (20 µL), AKT control lysate (100 µL), 5X Lysis Buffer 1 (1 mL), 10X TR-FRET Detection buffer (50 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-AKT pan (T308).
Assay Target Class: Kinase.
SwissProt: P31749 , P31751 , Q9Y243.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).