Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38α MAPK14 (T180/Y182) in cell lysates.
Components: Eu-phospho-p38a (5 µL), Acc-phospho-p38a (20 µL), p38a control lysate (100 µL), 5X Lysis Buffer 6 (1 mL), 10X TR-FRET Detection buffer (50 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-p38α (T180/Y182).
Assay Target Class: Kinase.
SwissProt: Q16539.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).