Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) in cell lysates.
Components: Eu-phospho-p53 (5 µL), Acc-phospho-p53 (20 µL), p53 control lysate (100 µL), 5X Lysis Buffer 2 (1 mL), 10X TR-FRET Detection buffer (50 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-p53 (S15).
Assay Target Class: Kinase.
SwissProt: P04637.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).