PIM3, Active, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay Conditions: Varying amounts of enzyme were incubated in 5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 4 mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA, 0.4 mM EDTA, 0.01 mM DTT, 0.05 mM ATP, 5 µg of RSK substrate and 32 µCi of [33P]-ATP was incubated at 30°C for 15 minutes. Then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Description: Recombinant humanserine/threonine-protein kinase PIM3(PIM3), GenBank Accession No.NM_145478, full-length with an N-terminalGST tag and expressed in a baculovirusinfectedSf9 insect cell expression system.MW = 63 kDa.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 10 mM glutathione, 0.1 mM EDTA,0.1 mM PMSF, 0.25 mM DTT, 25% glycerol

Genbank: NM_145478

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST tag

Uniprot: Q86V86

Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Fujii, C. et al., Int. J. Cancer. 2005; 114:: 209-218.
2. Li, Y.-Y., et al., Cancer Res. 2006; 66: 6741-6747.

• Artikelnummer: BPS41108
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 41108
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect