PKCθ (PKCtheta), GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: Protein Kinase C theta (PKCθ) is important component in the intracellular signaling cascade (1). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKCθ leading to defects in insulin signaling and glucose transport in skeletal muscle (2). Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKCθ is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes (2).

Description: Recombinant human PKCθ (PKCtheta), full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_006257

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q04759

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fas ligand-mediated apoptosis. J. Immunol. 2007;178(1):312-9.
2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004;114(6):823-7.

• Artikelnummer: BPS40165
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40165
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect