PKN2 (PRK2), GST-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: PKN2 (also known as PRK2) is a Rho effector and a member of the protein kinase C superfamily of serine/threonine kinases. PKN2 is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells (1). PKN2 is required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B, the phosphatase required for activation of mitotic cyclin/Cdk1 complexes at the G2/M transition. C-terminus of PKN2 could be a potential drug target for effector-specific pharmacological intervention of Rho-medicated biological processes (2).

Description: Recombinant human PKN2, also known as PRK2, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_006256

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q16513

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Schmidt A, et al: Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis. EMBO J. 2007 Mar 21;26(6):1624-36.
2. Lim W G, et al: The C-terminus of PRK2/PKNgamma is required for optimal activation by RhoA in a GTP-dependent manner. Arch Biochem Biophys. 2008 Nov 15;479(2):170-8.

• Artikelnummer: BPS40169
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40169
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect