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PLA2G4A, FLAG-His-tags Recombinant
PLA2G4A, FLAG-His-tags Recombinant
Artikelnummer
BPS60094
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1-749 (end)
Assay Conditions: PLA reaction was performed using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine substrate. Enzyme reaction is conducted in 100 µl volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl2, 0.5 mg/ml BSA, 10 µM substrate and 200 ng PLA2G4A. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature.
Description: Human PLA2G4A, GenBank Accession No. NM_024420, a.a. 1-749 (end), with C-terminal FLAG-His-tag, MW=87 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, and 20% glycerol.
Genbank: NM_024420
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-His-tags
Uniprot: P47712
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chalimoniuk M. Postepy Biochem. 2012; 58(2):204-8.
2. Nadalin S, Giacometti J, et al., Prostaglandins Leukot Essent Fatty Acids. 2013; 88(2):185-90.
Encompassing Amino Acids: 1-749 (end)
Assay Conditions: PLA reaction was performed using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine substrate. Enzyme reaction is conducted in 100 µl volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl2, 0.5 mg/ml BSA, 10 µM substrate and 200 ng PLA2G4A. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature.
Description: Human PLA2G4A, GenBank Accession No. NM_024420, a.a. 1-749 (end), with C-terminal FLAG-His-tag, MW=87 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, and 20% glycerol.
Genbank: NM_024420
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-His-tags
Uniprot: P47712
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chalimoniuk M. Postepy Biochem. 2012; 58(2):204-8.
2. Nadalin S, Giacometti J, et al., Prostaglandins Leukot Essent Fatty Acids. 2013; 88(2):185-90.
| Artikelnummer | BPS60094 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 60094 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
