PLA2G7 (Lp-PLA2), His-tag Recombinant

PLA2G7 (Lp-PLA2), His-tag Recombinant
Artikelnummer
BPS60091
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience

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Encompassing Amino Acids: 22-441(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted using liposomally incorporated Red/Green BODIPY PC-A2 (5 µM, Invitrogen) as a substrate in a reaction buffer (50 mM Tris, pH 8.8, 100 mM NaCl, 1 mM CaCl2). Reaction progress was monitored at ex480/em533 continuously at room temperature.

Description: Human PLA2G7, (GenBank Accession No. NM_005084), a.a. 22-441(end), with C-terminal His-tag, MW=49.2 kDa, expressed in a mammalian cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol

Genbank: NM_005084

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: Q13093

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Berg M., et al., Mol Cancer. 9:100. (2010).
2. Kopec AK., et al., Toxicol Appl Pharmacol.256(2):154-67. (2011).
Mehr Informationen
Artikelnummer BPS60091
Hersteller BPS Bioscience
Hersteller Artikelnummer 60091
Green Labware Nein
Verpackungseinheit 20 µg
Mengeneinheit STK
Wirt Human
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