PLK3 Polo Box Domain, MBP-tag, His-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 335-646

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: PLK3 or Polo-like kinase 3 is a cytokine-inducible kinase that is a member of the Polo family of serine/threonine protein kinases. PLK3 contains both a catalytic domain and a putative regulatory domain and plays an important role in the regulation of cell cycle progression and tumorigenesis. PLK3 is closely associated with centrosomes in several human cell lines and its localization is dependent on the integrity of microtubules. Hence, PLK3 is also involved in regulating microtubule dynamics and centrosomal function.

Description: Human N-terminal MBP tagged Plk3 polo box domain (GenBank Accession No.NM_004073/NP_004064), a.a. 335-646 with C-terminal His-tag, MW=80.5 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.

Genbank: NM_004073

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal MBP-tag, C-terminal His-tag

Uniprot: Q9H4B4

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Li, Z., et al., J. Biol. Chem. 280 (17), 16843-16850 (2005).
2.Xie,S., et al., Oncogene 23 (21), 3822-3829 (2004).

• Artikelnummer: BPS50302
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50302
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK